Journal: The Journal of Cell Biology

Article Title: Microglia control the glycinergic but not the GABAergic synapses via prostaglandin E2 in the spinal cord

doi: 10.1083/jcb.201607048

Figure Lengend Snippet: PGE2 mediates microglial regulation of synaptic GlyR accumulation via EP2 receptors. (A) Double labeling of organotypic slices showing Cox-2 and Iba1 IRs in control condition and after 30-min LPS application or 15-min 4AP application. Cox-2 IR is restricted to microglia. Bar, 20 µm. (B) Quantification (mean ± SEM) of Cox-2 fluorescence intensities over the corresponding Iba1 profiles in control (CT), LPS, and 4AP conditions. Circles represent single experiments. (C and D) The modulation of GlyR α1 QD synaptic dwell time (C) and stability (D) in control (CT), after LPS application (LPS), and after LPS and PF04418948 treatment (LPS + PF044). (C) Synaptic dwell time distributions indicating 25, 50 (black bar), and 75% of all trajectories. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kolmogorov-Smirnov test. (D) Percentage of stable synaptic trajectories detected during the imaging session ( n = 3 independent experiments; mean ± SEM; ns, P > 0.05; *, P < 0.05; t test). (E and F) Fluorescence intensities relative to control of synaptic GlyR α1 IR after application of LPS or 4AP in the presence of EP2 receptor antagonist PF04418948 (E) or the PKA antagonist H-89 (F) in organotypic slices. Mean ± SEM; circles represent single experiments.

Article Snippet: Slices were permeabilized and blocked in PBS 0.1% Triton X-100 and 0.25% fish gelatin and incubated with primary antibodies (anti-GlyR α1 [1:400; rabbit]; anti-GABA A R γ2 [1:50; Alomone Labs], anti-gephyrin [1:400; mAb7a; Synaptic Systems], anti-Cox-2 [1:200; M-19; Santa Cruz Biotechnology], anti-Iba1 [1:400; Wako], and anti-EP2R [Alomone Labs]).

Techniques: Labeling, Fluorescence, Imaging

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: The pharmaceutical applications of total flavonoids extract from Isatis tinctoria L. leaves

doi: 10.1016/j.prmcm.2022.100122

Figure Lengend Snippet: Fig. 8. Effects of TFE from Folium isatidis on the genes expression of TLR4 (a), TRAF6 (b), TRIF (c), I 𝜅B (d) and p65 (e) in lungs tissues of LPS induced ALI mice ( # compared with the control, ∗ compared with LPS, ∗ P < 0.05, ∗ ∗ / ## P < 0.01).

Article Snippet: Antibodies against TLR4, RAF6, TRIF, NF- κB p65, p-NF- κB p65, I κB α, p-I κB α and β-actin were urchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and econdary antibodies were obtained from Wuhan Boster Biological Techology., Ltd (Wuhan, China).

Techniques: Expressing, Control

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: The pharmaceutical applications of total flavonoids extract from Isatis tinctoria L. leaves

doi: 10.1016/j.prmcm.2022.100122

Figure Lengend Snippet: Fig. 9. Effects of TFE from Folium isatidis on the proteins expression of TLR4 (a), TRAF6 (b), TRIF (c), I 𝜅B (d), p-I 𝜅B (e), p65 (f) and p-p65 (g) in lungs tissues of LPS induced ALI mice ( # compared with the cntrol, ∗ compared with LPS, ∗ P < 0.05, ∗ ∗ / ## P < 0.01).

Article Snippet: Antibodies against TLR4, RAF6, TRIF, NF- κB p65, p-NF- κB p65, I κB α, p-I κB α and β-actin were urchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and econdary antibodies were obtained from Wuhan Boster Biological Techology., Ltd (Wuhan, China).

Techniques: Expressing

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: The pharmaceutical applications of total flavonoids extract from Isatis tinctoria L. leaves

doi: 10.1016/j.prmcm.2022.100122

Figure Lengend Snippet: Fig. 10. Regulation of TFE from Folium isatidis on TLR4-TRIF-NF- 𝜅B signaling pathway in LPS induced ALI mice.

Article Snippet: Antibodies against TLR4, RAF6, TRIF, NF- κB p65, p-NF- κB p65, I κB α, p-I κB α and β-actin were urchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and econdary antibodies were obtained from Wuhan Boster Biological Techology., Ltd (Wuhan, China).

Techniques:

Journal: BMC Cancer

Article Title: Identification of a Novel CD8 + T cell exhaustion-related gene signature for predicting survival in hepatocellular carcinoma

doi: 10.1186/s12885-023-11648-x

Figure Lengend Snippet: Expression of STAM, ANXA5 and MAD2L2 in HCC cell lines. A The expressions of STAM, ANXA5 and MAD2L2 in normal hepatocytes and hepatoma cell lines were detected using Western blotting. Knocking down STAM, ANXA5 and MAD2L2 inhibited the proliferation and migration of HCC cells. B The knockdown efficiency of STAM, ANXA5 and MAD2L2 was detected using Western Blotting. C Plate cloning experiment showed that the number of cloned cell clusters formed by hepatocellular carcinoma cells after knockdown of STAM, ANXA5 and MAD2L2 was significantly reduced; D The results of CCK8 experiment showed that inhibiting the expression of STAM, ANXA5 and MAD2L2 decreased the proliferative ability of HCC cells; E Scratch assay showed that knockdown of STAM, ANXA5 and MAD2L2 inhibited the migration of hepatocellular carcinoma cells; F Transwell experiment showed that the migration ability of hepatocellular carcinoma cells was weakened after inhibiting the expression of STAM, ANXA5 and MAD2L2 .* P < 0.05, ** P < 0.01, *** P < 0.001, there was no significant difference in ns

Article Snippet: Anti- MAD2L2 (BM5428) and anti- STAM (A00864-1) antibodies were purchased from BosterBio (Pleasanton, CA, USA), while Anti- TBL1XR1 (MBS850368), Anti- ANXA5 (MBS474163), Anti- FKBP1A (MBS9404059) and Anti- PPM1G (MBS626576) antibodies were purchased from MyBioSource (San Diego, CA, USA).

Techniques: Expressing, Western Blot, Migration, Knockdown, Cloning, Clone Assay, Wound Healing Assay